Virginia Tech® home

Metabolism Core

Mitochondrial Bioenergetics
assess oxygen consumption and CO2 production for measures of state 3 (ADP- stimulated), state 4 respiration, and uncoupled (FCCP- stimulated) respiration in both cultured cells, mitochondria isolated from various tissues, and isolated skeletal muscle fibers.

In-Vivo / In-Vitro Substrate Metabolism
assess substrate metabolism in cell culture models, tissue homogenates, and/or isolated mitochondria utilizing radiolabeled substrates.

Whole body Calorimetry
TSE LabMaster System is a modular system for metabolic and behavioral monitoring in mice (three day experiment)

Glucose and Insulin Tolerance Test (GTT and ITT)
Mice are fasted before the test (usually overnight for GTT and four hours for ITT)

  • A droplet of blood will be collected from tail vein as time point 0.
  • Pharmaceutical grade of either dextrose solution (2g/kg) or human porcine insulin (1U/kg) will be injected i.p.(intraperitoneal) to mice based on the individual body weight.
  • Blood samples will be collected after injection at 15min, 30min, 60min, 90min, and 120min for GTT; or at 15min, 30min, 45min, and 60min for ITT.
  • Blood glucose will be measured by glucometer.